Journal: Redox Biology

Article Title: Differentiation of intestinal stem cells toward goblet cells under systemic iron overload stress are associated with inhibition of Notch signaling pathway and ferroptosis

doi: 10.1016/j.redox.2024.103160

Figure Lengend Snippet: List of antibodies used in Western blot analysis and immunofluorescence microscopy.

Article Snippet: Anti-TfR , Bioss , bs-0988R , WB.

Techniques: Western Blot, Immunofluorescence, Microscopy

Journal: Experimental and Therapeutic Medicine

Article Title: In vitro culture and biological properties of broiler adipose-derived stem cells

doi: 10.3892/etm.2018.6445

Figure Lengend Snippet: Sequences of primers used for polymerase chain reaction.

Article Snippet: The following reagents were used in the present study: Dulbecco's modified Eagle's medium (DMEM)/F12, fetal bovine serum (FBS), L-glutamine, and penicillin and streptomycin (all from Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA) trypsin (dilution, 1:250) and 0.02% (w/v) EDTA (Amresco, Inc., Framingham, MA, USA), rabbit anti-chicken CD29 (bs-0486R), CD44 (bs-2507R), CD71 (bs-1782R) and CD73 (bs-4834R) polyclonal primary antibody, goat serum, EDTA, fluorescein isothiocyanate (FITC)-conjugated goat-anti-rabbit immunoglobulin (Ig)G (all from Bioss, Beijing, China), ascorbic acid sodium salt, dexamethasone, hepatocyte growth factor, indometacin, insulin transferrin-selenium, 3-isobutyl-1-methylxanthine (IBMX), β-glycerophosphate, oil red O, collagenase I (all from Sigma-Aldrich; Merck KGaA, Darmstadt, Germany), basic fibroblast growth factor (bFGF), fibroblast growth factor-4 (FGF-4; both from Peprotech, Inc., Rocky Hill, NJ, USA), alizarin red (Boster, Wuhan, China) and TRIzol (Invitrogen; Thermo Fisher Scientific, Inc.).

Techniques: Sequencing

Journal: Experimental and Therapeutic Medicine

Article Title: In vitro culture and biological properties of broiler adipose-derived stem cells

doi: 10.3892/etm.2018.6445

Figure Lengend Snippet: Surface markers of the ADSCs. Surface markers of the ADSCs were similar to those of bone marrow mesenchymal stem cells. The expression of CD29, CD31, CD44, CD71 and CD73 was detected by RT-PCR and immunofluorescence. (A) RT-PCR analysis indicated that the ADSCs expressed CD29, CD44, CD71 and CD73, but not CD31. GADPH was used as an internal control. (B) Immunofluorescence demonstrated immunopositivity for CD29, CD44, CD71 and CD73 (scale bars, 50 µm). ADSCs, adipose-derived stem cells; RT-PCR, reverse-transcription polymerase chain reaction analysis; P3, passage 3.

Article Snippet: The following reagents were used in the present study: Dulbecco's modified Eagle's medium (DMEM)/F12, fetal bovine serum (FBS), L-glutamine, and penicillin and streptomycin (all from Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA) trypsin (dilution, 1:250) and 0.02% (w/v) EDTA (Amresco, Inc., Framingham, MA, USA), rabbit anti-chicken CD29 (bs-0486R), CD44 (bs-2507R), CD71 (bs-1782R) and CD73 (bs-4834R) polyclonal primary antibody, goat serum, EDTA, fluorescein isothiocyanate (FITC)-conjugated goat-anti-rabbit immunoglobulin (Ig)G (all from Bioss, Beijing, China), ascorbic acid sodium salt, dexamethasone, hepatocyte growth factor, indometacin, insulin transferrin-selenium, 3-isobutyl-1-methylxanthine (IBMX), β-glycerophosphate, oil red O, collagenase I (all from Sigma-Aldrich; Merck KGaA, Darmstadt, Germany), basic fibroblast growth factor (bFGF), fibroblast growth factor-4 (FGF-4; both from Peprotech, Inc., Rocky Hill, NJ, USA), alizarin red (Boster, Wuhan, China) and TRIzol (Invitrogen; Thermo Fisher Scientific, Inc.).

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Immunofluorescence, Derivative Assay

Journal: Experimental and Therapeutic Medicine

Article Title: In vitro culture and biological properties of broiler adipose-derived stem cells

doi: 10.3892/etm.2018.6445

Figure Lengend Snippet: Flow cytometric analysis of ADSCs. Cell surface antigens of the ADSCs were similar to those of bone marrow mesenchymal stem cells. The expression of (A) control, (B) CD29, (C) CD44, (D) CD71 and (E) CD73 was detected by flow cytometry. The results indicated that ADSCs at P5 exhibited high expression of CD29, CD44, CD71 and CD73. ADSCs, adipose-derived stem cells; P5, passage 5.

Article Snippet: The following reagents were used in the present study: Dulbecco's modified Eagle's medium (DMEM)/F12, fetal bovine serum (FBS), L-glutamine, and penicillin and streptomycin (all from Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA) trypsin (dilution, 1:250) and 0.02% (w/v) EDTA (Amresco, Inc., Framingham, MA, USA), rabbit anti-chicken CD29 (bs-0486R), CD44 (bs-2507R), CD71 (bs-1782R) and CD73 (bs-4834R) polyclonal primary antibody, goat serum, EDTA, fluorescein isothiocyanate (FITC)-conjugated goat-anti-rabbit immunoglobulin (Ig)G (all from Bioss, Beijing, China), ascorbic acid sodium salt, dexamethasone, hepatocyte growth factor, indometacin, insulin transferrin-selenium, 3-isobutyl-1-methylxanthine (IBMX), β-glycerophosphate, oil red O, collagenase I (all from Sigma-Aldrich; Merck KGaA, Darmstadt, Germany), basic fibroblast growth factor (bFGF), fibroblast growth factor-4 (FGF-4; both from Peprotech, Inc., Rocky Hill, NJ, USA), alizarin red (Boster, Wuhan, China) and TRIzol (Invitrogen; Thermo Fisher Scientific, Inc.).

Techniques: Expressing, Flow Cytometry, Derivative Assay

Journal: Toxics

Article Title: In Vitro Evaluation of the Effects of Cadmium on Endocytic Uptakes of Proteins into Cultured Proximal Tubule Epithelial Cells

doi: 10.3390/toxics8020024

Figure Lengend Snippet: Fluorescence imaging of endocytosed proteins in mouse proximal tubule epithelial cell (PTEC)-derived S1 cells. ( A ) S1 cells were incubated with 50 µg/mL fluorescein isothiocyanate (FITC)-albumin, Alexa-transferrin, FITC-β 2 -MG, or FITC-MT (green) for 30 min and then fixed with paraformaldehyde for immunofluorescence labeling with anti-EEA1 (red). Yellow staining demonstrates the colocalization of fluorescent-labeled proteins and early endosomes. ( B ) S1 cells were incubated with Alexa-transferrin for 1, 5, 10, 15, and 30 min. The localization of Alexa-transferrin (green) and early endosomes stained with anti-EEA1 (red) was visualized by confocal microscopy. Bars, 5 µm.

Article Snippet: Rabbit anti-transferrin receptor polyclonal antibody was purchased from Abnova (Taipei, Taiwan).

Techniques: Fluorescence, Imaging, Derivative Assay, Incubation, Immunofluorescence, Labeling, Staining, Confocal Microscopy

Journal: Toxics

Article Title: In Vitro Evaluation of the Effects of Cadmium on Endocytic Uptakes of Proteins into Cultured Proximal Tubule Epithelial Cells

doi: 10.3390/toxics8020024

Figure Lengend Snippet: Effects of Cd on the endocytosis efficiencies of albumin and transferrin into S1 cells. S1 cells were exposed to CdCl 2 for 1, 3, and 6 days and then incubated with FITC-albumin ( A ) or Alexa-transferrin ( B ) for 30 min. The endocytosis efficiencies were determined by flow cytometry and expressed as percentages of the control cells (no exposure to Cd). Data are presented as means ± SD ( n = 3–4).

Article Snippet: Rabbit anti-transferrin receptor polyclonal antibody was purchased from Abnova (Taipei, Taiwan).

Techniques: Incubation, Flow Cytometry, Control

Journal:

Article Title: The effects of iron deficiency on lymphocyte cytokine production and activation: preservation of hepatic iron but not at all cost

doi: 10.1046/j.1365-2249.2001.01707.x

Figure Lengend Snippet: Characteristics of children tested for serum transferrin receptor levels, by level

Article Snippet: Serum transferrin receptor levels Levels were assessed using an enzyme-linked immunosorbent assay with polyclonal and monoclonal antibodies against purified transferrin receptor (Ramco Laboratories, Inc., Houston, TX, USA) [ 14 , 16 ].

Techniques: Expressing

Journal:

Article Title: The effects of iron deficiency on lymphocyte cytokine production and activation: preservation of hepatic iron but not at all cost

doi: 10.1046/j.1365-2249.2001.01707.x

Figure Lengend Snippet: Cell-specific cytokines related to the presence or absence of iron deficiency. Iron deficiency was defined as being present when the serum transferrin receptor level was ≥10 μg/ml. Boxes include medians (lines) and values between the 25th and 75th percentiles. Lines extend to the furthest value within ±1·5 × the interquartile range from the 25th and 75th percentiles; outliers are presented as circles.

Article Snippet: Serum transferrin receptor levels Levels were assessed using an enzyme-linked immunosorbent assay with polyclonal and monoclonal antibodies against purified transferrin receptor (Ramco Laboratories, Inc., Houston, TX, USA) [ 14 , 16 ].

Techniques:

Journal:

Article Title: The effects of iron deficiency on lymphocyte cytokine production and activation: preservation of hepatic iron but not at all cost

doi: 10.1046/j.1365-2249.2001.01707.x

Figure Lengend Snippet: Percentage of lymphocytes producing induced IL-4, by the presence or absence of severe iron deficiency. Severe iron deficiency was defined as being present when the serum transferrin receptor level was ≥30 μg/ml. Individual data points are provided for those with severe iron deficiency (n = 7). For the non-deficient, the box includes the median (line) and values between the 25th and 75th percentile. Lines extend to the furthest value within ±1·5 × the interquartile range from the 25th and 75th percentiles; outliers are presented as circles.

Article Snippet: Serum transferrin receptor levels Levels were assessed using an enzyme-linked immunosorbent assay with polyclonal and monoclonal antibodies against purified transferrin receptor (Ramco Laboratories, Inc., Houston, TX, USA) [ 14 , 16 ].

Techniques:

Journal:

Article Title: The effects of iron deficiency on lymphocyte cytokine production and activation: preservation of hepatic iron but not at all cost

doi: 10.1046/j.1365-2249.2001.01707.x

Figure Lengend Snippet: Activation markers related to the presence or absence of iron deficiency, by cell type. Iron deficiency was defined as being present when the serum transferrin receptor level was ≥10 μg/ml. Boxes include medians (lines) and values between the 25th and 75th percentiles. Lines extend to the furthest value within ±1·5 × the interquartile range from the 25th and 75th percentiles; outliers are presented as circles.

Article Snippet: Serum transferrin receptor levels Levels were assessed using an enzyme-linked immunosorbent assay with polyclonal and monoclonal antibodies against purified transferrin receptor (Ramco Laboratories, Inc., Houston, TX, USA) [ 14 , 16 ].

Techniques: Activation Assay